Acta Chir Orthop Traumatol Cech. 2018; 85(5):351-358 | DOI: 10.55095/achot2018/060
Immunohistochemical Detection of Mast and Dendritic Cells in Periprosthetic Tissues of Aseptically Loosened Total ProsthesesOriginal papers
- 1 Ústav patologie Lékařské fakulty Ostravské univerzity a Fakultní nemocnice Ostrava
- 2 Ortopedická klinika Lékařské fakulty Univerzity Palackého v Olomouci a Fakultní nemocnice Olomouc
- 3 CGB laboratoř, a.s., Ostrava
- 4 Oddělení anorganické analýzy, Centrum nanotechnologií, Vysoká škola báňská - Technická univerzita Ostrava
PURPOSE OF THE STUDY:
This study deals with the possibilities and application of immunohistochemical methods to detect mast and dendritic cells in periprosthetic tissues in patients with aseptically loosened total joint replacements of the knee and hip. The purpose of the study was to quantify and characterize the distribution of mast and dendritic cells in the examined samples and to study the statistically significant relations between the aforementioned cell populations and selected parameters characterizing the patients, implants or tissue response. Based on the proved findings, a possible relation between mast and dendritic cells and histomorphological patterns of aseptic loosening and the benefit of the applied immunohistochemical methods was evaluated.
MATERIAL AND METHODS:
Periprosthetic tissues from a total of 31 patients (17 patients after a revision surgery of hip prosthesis, 14 patients after a revision surgery of knee prosthesis) were examined. The collected samples were processed according to the standard protocol for the purposes of histological and immunochemical examination. Antibodies against tryptase and CD117 were used for immunohistochemical detection of mast cells. Dendritic cells were detected by means of S100 and CD1a antibodies. Quantification of both the cell populations was carried out by optical microscopy in 20 high power fields at 400-times magnification. From among the applied methods we picked the more sensitive one for statistical evaluation. It was tryptase in the case of mast cells and S100 in the case of dendritic cells.
RESULTS:
Mast and dendritic cells were mostly distributed dispersively in periprosthetic tissues; however, they also occurred in groups perivasally or near necrotic parts. The examined samples showed the presence of 60 mast cells and 50 dendritic cells on average. The increased density of mast and dendritic cells was associated with polypously formed pseudosynovium and cement fixation of prostheses; this relation was statistically significant. It was impossible to prove the correlation between the quantity of the observed cell populations and the nature and the number of the observed particles because wear particles were present dispersely in all the samples. Another statistically significant relation to the type of material or implant fixation or other examined histomorphological patterns was not proved. A strong density of mast cells with a minimum presence of dendritic cells was observed in the control patient group.
DISCUSSION:
The differences in density of S100 positive dendritic cells between the control and examined group of patients can be caused by the activation of dendritic cells by exogenous or endogenous pathways of immune processes going on after the implantation of endoprosthesis. The statistically significant interrelation of mast cells, polypously formed pseudosynovium and cement wear particles can be explained at least in part as a tissue reaction induced by cement particles.
CONCLUSIONS:
We proved the presence of two immunologically significant cell populations in periprosthetic tissues. The said findings indicate a conclusion of significant functional participation of mast and dendritic cells in pathogenesis of aseptic loosening and periprosthetic osteolysis. Nevertheless, this will have to be proved in another way and with the use of another method.
Keywords: dendritic cells, mast cells, aseptic loosening, total joint replacement, immune reaction, adverse reaction
Published: October 1, 2018 Show citation
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References
- Breton G, Lee J, Liu K, Nussenzweig MC. Defining human dendritic cell progenitors by multiparametric flow cytometry. Nat Protoc. 2015;10:1407-1422.
Go to original source...
Go to PubMed...
- Cildir G, Pant H, Lopez AF, Tergaonkar V. The transcriptional program, functional heterogeneity, and clinical targeting of mast cells. J Exp Med. 2017;214:2491-2506.
Go to original source...
Go to PubMed...
- Dyskova T, Gallo J, Kriegova E. The role of the chemokine system in tissue response to prosthetic by-products leading to periprosthetic osteolysis and aseptic loosening. Front Immunol. 2017;8:1026.
Go to original source...
Go to PubMed...
- Galdiero MR, Varricchi G, Seaf M, Marone G, Levi-Schaffer F, Marone G. Bidirectional mast cell-eosinophil interactions in inflammatory disorders and cancer. Front Med (Lausanne). 2017;4:103.
Go to original source...
Go to PubMed...
- Gallo J, Luzna P, Holinka M, Ehrmann J, Zapletalová J, Lošťák J. [Validity of the Morawietz classification for evaluation of periprosthetic tissue]. Acta Chir Orthop Traumatol Cech. 2015;82:126-134.
Go to original source...
Go to PubMed...
- Gallo J, Raska M, Kriegova E, Goodman SB. Inflammation and its resolution and the musculoskeletal system. J Orthop Translat. 2017;10:52-67.
Go to original source...
Go to PubMed...
- Goodman SB, Huie P, Song Y, Schurman D, Maloney W, Woolson S, Sibley R. Cellular profile and cytokine production at prosthetic interfaces. Study of tissues retrieved from revised hip and knee replacements. J Bone Joint Surg Br. 1998;80:531-539.
Go to original source...
- Gu Q, Shi Q, Yang H. The role of TLR and chemokine in wear particle-induced aseptic loosening. J Biomed Biotechnol. 2012;2012:596870.
Go to original source...
Go to PubMed...
- Guilarte M, Sala-Cunill A, Luengo O, Labrador-Horrillo M, Cardona V. The mast cell, contact, and coagulation system connection in anaphylaxis. Front Immunol. 2017;8:846.
Go to original source...
Go to PubMed...
- Gurish MF, Austen KF. Developmental origin and functional specialization of mast cell subsets. Immunity. 2012;37:25-33.
Go to original source...
Go to PubMed...
- Hallab NJ, Jacobs JJ. Chemokines associated with pathologic responses to orthopedic implant debris. Front Endocrinol (Lausanne). 2017;8:5.
Go to original source...
Go to PubMed...
- Imai S, Konttinen YT, Jumppanen M, Lindy O, Ceponis A, Kemppinen P, Sorsa T, Santavirta S, Xu JW, Lopez-Otin C. High levels of expression of collagenase-3 (MMP-13) in pathological conditions associated with a foreign-body reaction. J Bone Joint Surg Br. 1998;80:701-710.
Go to original source...
- Konttinen YT, Takagi M, Mandelin J, Lassus J, Salo J, Ainola M, Li TF, Virtanen I, Liljestrom M, Sakai H, Kobayashi Y, Sorsa T, Lappalainen R, Demulder A, Santavirta S. Acid attack and cathepsin K in bone resorption around total hip replacement prosthesis. J Bone Miner Res. 2001;16:1780-1786.
Go to original source...
Go to PubMed...
- Kou PM, Babensee JE. Macrophage and dendritic cell phenotypic diversity in the context of biomaterials. J Biomed Mater Res A. 2011;96:239-260.
Go to original source...
Go to PubMed...
- Lahdeoja T, Pajarinen J, Kouri VP, Sillat T, Salo J, Konttinen YT. Toll-like receptors and aseptic loosening of hip endoprosthesis-a potential to respond against danger signals? J Orthop Res. 2010;28:184-190.
Go to original source...
Go to PubMed...
- Landgraeber S, Loer F, Heep H, Classen T, Grabellus F, Totsch M, von Knoch M. Tartrate-resistant acid phosphatase 5b and C-terminal telopeptides of type I collagen as markers for diagnosis of aseptic loosening after total hip replacement. Arch Orthop Trauma Surg. 2010;130:441-445.
Go to original source...
Go to PubMed...
- Lennox DW, Schofield BH, McDonald DF, Riley LH, Jr. A histologic comparison of aseptic loosening of cemented, press-fit, and biologic ingrowth prostheses. Clin Orthop Relat Res. 1987:171-191.
Go to original source...
- Maitra R, Clement CC, Scharf B, Crisi GM, Chitta S, Paget D, Purdue PE, Cobelli N, Santambrogio L. Endosomal damage and TLR2 mediated inflammasome activation by alkane particles in the generation of aseptic osteolysis. Mol Immunol. 2009;47:175-184.
Go to original source...
Go to PubMed...
- Marichal T, Tsai M, Galli SJ. Mast cells: potential positive and negative roles in tumor biology. Cancer Immunol Res. 2013;1:269-279.
Go to original source...
Go to PubMed...
- Nich C, Takakubo Y, Pajarinen J, Ainola M, Salem A, Sillat T, Rao AJ, Raska M, Tamaki Y, Takagi M, Konttinen YT, Goodman SB, Gallo J. Macrophages-key cells in the response to wear debris from joint replacements. J Biomed Mat Res A. 2013;101:3033-3045.
Go to original source...
Go to PubMed...
- Perino G, Ricciardi BF, Jerabek SA, Martignoni G, Wilner G, Maass D, Goldring SR, Purdue PE. Implant based differences in adverse local tissue reaction in failed total hip arthroplasties: a morphological and immunohistochemical study. BMC Clin Pathol. 2014;14:39.
Go to original source...
Go to PubMed...
- Qu Z, Liebler JM, Powers MR, Galey T, Ahmadi P, Huang XN, Ansel JC, Butterfield JH, Planck SR, Rosenbaum JT. Mast cells are a major source of basic fibroblast growth factor in chronic inflammation and cutaneous hemangioma. Am J Pathol. 1995;147:564-573.
- Ray P, Krishnamoorthy N, Oriss TB, Ray A. Signaling of c-kit in dendritic cells influences adaptive immunity. Ann NY Acad Sci. 2010;1183:104-122.
Go to original source...
Go to PubMed...
- Ricciardi BF, Nocon AA, Jerabek SA, Wilner G, Kaplowitz E, Goldring SR, Purdue PE, Perino G. Histopathological characterization of corrosion product associated adverse local tissue reaction in hip implants: a study of 285 cases. BMC Clin Pathol. 2016;16:3.
Go to original source...
Go to PubMed...
- Shanbhag AS, Kaufman AM, Hayata K, Rubash HE. Assessing osteolysis with use of high-throughput protein chips. J Bone Joint Surg Am. 2007;89:1081-1089.
Go to original source...
Go to PubMed...
- Solovieva SA, Ceponis A, Konttinen YT, Takagi M, Suda A, Eklund KK, Sorsa T, Santavirta S. Mast cells in loosening of totally replaced hips. Clin Orthop Relat Res. 1996;322:158-165.
Go to original source...
- Thwe PM, Amiel E. The role of nitric oxide in metabolic regulation of dendritic cell immune function. Cancer Lett. 2017;412:236-242.
Go to original source...
Go to PubMed...
- Tomankova T, Kriegova E, Fillerova R, Luzna P, Ehrmann J, Gallo J. Comparison of periprosthetic tissues in knee and hip joints: differential expression of CCL3 and DC-STAMP in total knee and hip arthroplasty and similar cytokine profiles in primary knee and hip osteoarthritis. Osteoarthr Cartilage. 2014;22:1851-1860.
Go to original source...
Go to PubMed...
- Vitte F, Fabiani B, Benet C, Dalac S, Balme B, Delattre C, Vergier B, Beylot-Barry M, Vignon-Pennamen D, Ortonne N, Algros MP, Carlotti A, Samaleire D, Frouin E, Levy A, Laroche L, Theate I, Monnien F, Mugneret F, Petrella T. Specific skin lesions in chronic myelomonocytic leukemia: a spectrum of myelomonocytic and dendritic cell proliferations: a study of 42 cases. Am J Surg Pathol. 2012;36:1302-1316.
Go to original source...
Go to PubMed...
- Whitehouse MR, Endo M, Zachara S, Nielsen TO, Greidanus NV, Masri BA, Garbuz DS, Duncan CP. Adverse local tissue reactions in metal-on-polyethylene total hip arthroplasty due to trunnion corrosion: the risk of misdiagnosis. Bone Joint J. 2015;97-B:1024-1030.
Go to original source...
Go to PubMed...
- Willert HG, Buchhorn GH, Fayyazi A, Flury R, Windler M, Koster G, Lohmann CH. Metal-on-metal bearings and hypersensitivity in patients with artificial hip joints. A clinical and histomorphological study. J Bone Joint Surg Am. 2005;87:28-36.
Go to original source...
Go to PubMed...
- Wu DD, Li T, Ji XY. Dendritic Cells in Sepsis: Pathological Alterations and Therapeutic Implications. J Immunol Res. 2017;2017:3591248.
Go to original source...
Go to PubMed...
- Zhan HX, Zhou B, Cheng YG, Xu JW, Wang L, Zhang GY, Hu SY. Crosstalk between stromal cells and cancer cells in pancreatic cancer: New insights into stromal biology. Cancer Lett. 2017;392:83-93.
Go to original source...
Go to PubMed...